Application

Research Sub CategorySecondary Antibodies Adsorbed for Dual LabelingFragment Specific Secondary Antibodies

ELISA: 1:4,000-1:8,000

Optimal working dilutions must be determined by the end user.

Goat anti-Mouse IgG γ chain Antibody, HRP conjugate, Species Adsorbed is an antibody against Mouse IgG γ chain for use in ELISA.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Immunogen

Prepared from purified mouse IgG γ chain. Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.

Epitope: IgG γ chain

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Liquid in 50% glycerol/50% PBS, pH 7.4.

Affinity Chromatography

Specificity

Reacts with heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3. Absorbed for mouse IgM and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.